Lohmann Lab · Centre for Organismal Studies, Heidelberg University. Access is restricted to lab members.
Meristemania3D
SAM single-cell expression explorer · V.84
Segmented SAM from Christian Wenzl
stage-resolved: young vs old primordia painted from matched scRNA maturation stage
Markers
Activate for cell-to-cell comparison. Deactivate for maximum per-cell sensitivity.
Slice: use the slider inside each view — orbit to any angle, then drag to cut away cells between you and the interior. Each view slices independently.
Coverage & caveats
This is an apical confocal SAM specimen. Expression is projected from matched scRNA-seq pools (Xu 2025 ap1/cal), not measured in situ. ~49% of sequenced cells map to no zone here and are excluded from the projection:
Photosynthetic / differentiated (~2,840 cells) — no differentiated tissue in this dome.
Basal rib & basal PZ (~2,940 cells) — deep zones absent from this apical section; deliberately excluded.
Deeper peripheral meristem — only the apical PZ subset is represented.
Low-complexity / unknown (~245 cells) — no clear identity.
WUS/CLV3 are validated against the measured FP reporters (Mean_wus/Mean_clv3); niche pools carry only marker-flagged cells. FP reporters diffuse laterally, so mRNA zones are tighter than the reporter halo.
Ask (natural language)
Type a question or command in plain English — e.g. show WUS, which zone has the most KNOLLE, genes similar to STM, turn on depth correction, spin the side view, slice the top view deeper, export similar genes.
Command channel (programmatic / agent)
Paste JSON and run. Schema: {"gene":"WUS"} paints one gene; {"genes":[...]} loads a stepper list; add "scale":"absolute" to set the colour scale.