Method development is part of how we work. From the community-standard GreenGate cloning system to interactive explorers of single-cell expression data in shoot and root meristems.
GreenGate is a Golden Gate–based system for rapidly assembling plant transformation constructs. It uses a single type IIS restriction enzyme and just six classes of insert module — plant promoter, N‑terminal tag, coding sequence, C‑terminal tag, plant terminator and plant resistance cassette — and lets several expression cassettes be stacked into one binary destination vector from a library of pre-cloned building blocks. It is cheap, reliable, and now used by plant labs worldwide.
Original system — GreenGate
The original modular system for rapid, cheap and reliable assembly of plant transformation constructs. The complete set of GreenGate entry and destination vectors is distributed as a kit through Addgene, with maps, sequences and documentation for every module — request the plasmids directly for use in your own transformation pipeline.
Extension — GreenGate 2.0
An important, backwards-compatible extension developed by the neighbouring Maizel lab at COS Heidelberg. GreenGate 2.0 adds one-pot entry-clone generation (a Universal Entry Generator, pUEG), up to twelve Level 0 modules per transcriptional unit, the GreenBraid method for iterative stacking of multigene constructs, and ready-made modules for inducible and fluorescent markers — while remaining compatible with most existing GreenGate modules.
To make our single-cell and single-nucleus transcriptome data explorable at the bench, we build interactive viewers that let you paint gene expression directly onto the tissue. These tools are currently provided for lab-internal use only and are protected by lab credentials.
Shoot apical meristem — ready
An interactive 3D viewer of a segmented Arabidopsis shoot apical meristem. Query any gene by symbol or AGI to paint its expression cell-by-cell across the reconstructed meristem, step through gene lists, and find genes with similar spatial patterns — with young and old primordia painted from their matched scRNA maturation stage. Segmented SAM by Christian Wenzl.
Open explorer
Root tip — ready
An interactive 2D viewer of the Arabidopsis root tip. Query any gene by symbol or AGI to paint its expression across the cell types of the root — from the stem cell niche and columella through the meristematic and elongation zones — directly from single-cell RNA-seq. Select from seven independent datasets.
Open explorer
Further datasets and viewers are added here as they are released.
